CDK4 antibody (Cyclin-Dependent Kinase 4) (AA 211-304)

Details for Product anti-CDK4 Antibody No. ABIN393883, Supplier: Log in to see
Antigen
  • zgc:153726
  • CMM3
  • PSK-J3
  • Crk3
  • 8-6
  • CDK4
  • CDK4/6
  • CG5072
  • Cdk4/6
  • DmCdk4
  • Dmel\\CG5072
  • Pk53C
  • Pk?7
  • cdk
  • cdk4
  • cdk4/6
  • dCdk4
  • l(2)05428
  • l(2)0671
  • l(2)k06503
  • l(2)s4639
  • l(2)sh0671
  • xcdk4
  • cyclin-dependent kinase 4
  • Cyclin-dependent kinase 4
  • cdk4
  • CDK4
  • Cdk4
Alternatives
anti-Human CDK4 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 211-304
52
45
15
14
12
10
10
9
9
8
8
8
6
5
5
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
285
136
114
21
11
7
6
6
6
1
1
1
Host
Mouse
171
138
5
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CDK4 antibody is un-conjugated
13
13
10
7
6
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
276
120
66
65
56
24
24
23
13
7
6
6
5
3
2
2
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 8A10
Isotype IgG1 kappa
Specificity CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CDK4 (CDK4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 33730 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Pathways Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody (ABIN393883) Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody (ABIN393883) anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody (Image 2)
Background publications Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Zeng, Shaikh, Harrison, Adon, Trimboli, Carroll, Sharma, Timmers, Chodosh, Leone, Saavedra: "The Ras oncogene signals centrosome amplification in mammary epithelial cells through cyclin D1/Cdk4 and Nek2." in: Oncogene, Vol. 29, Issue 36, pp. 5103-12, 2010 (PubMed).

Wiedemeyer, Dunn, Quayle, Zhang, Chheda, Dunn, Zhuang, Rosenbluh, Chen, Xiao, Shapiro, Hahn, Chin: "Pattern of retinoblastoma pathway inactivation dictates response to CDK4/6 inhibition in GBM." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11501-6, 2010 (PubMed).

Liu, Wu, Chen, Ter-Minassian, Asomaning, Zhai, Wang, Su, Heist, Kulke, Lin, Liu, Christiani: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Michaud, Solomon, Oermann, Kim, Zhong, Prados, Ozawa, James, Waldman: "Pharmacologic inhibition of cyclin-dependent kinases 4 and 6 arrests the growth of glioblastoma multiforme intracranial xenografts." in: Cancer research, Vol. 70, Issue 8, pp. 3228-38, 2010 (PubMed).

Did you look for something else?