Cyclin-Dependent Kinase 4 (CDK4) antibody

Antigen: Cyclin-Dependent Kinase 4 (CDK4)

Synonyms: CMM3, PSK-J3, MGC14458, 8-6, CDK4, CDK4/6, DmCdk4, Pk53C, Pk?7, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, DmelCG5072, CG5072, MGC133903

Clonality: Monoclonal (8A10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393883

Additional Information

Alternative name: CDK4

Gene ID: 1019

Immunogen: CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 8A10

Description: Other names: CMM3, MGC14458, PSKJ3 cyclin-dependent kinase 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33730 DA

Comments:

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to CDK4 (AT1476a) on HeLa cell . [antibody concentration 10 ug/ml] CDK4 monoclonal antibody (M09), clone 8A10 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).

anti-Cyclin-Dependent Kinase 4 (CDK4) antibody (Image 2)

Publications

Product: Michaud, Solomon, Oermann et al.: "Pharmacologic inhibition of cyclin-dependent kinases 4 and 6 arrests the growth of glioblastoma multiforme intracranial xenografts." in: Cancer research, Vol. 70, Issue 8, pp. 3228-38, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Wiedemeyer, Dunn, Quayle et al.: "Pattern of retinoblastoma pathway inactivation dictates response to CDK4/6 inhibition in GBM." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11501-6, 2010 (PubMed).

Zeng, Shaikh, Harrison et al.: "The Ras oncogene signals centrosome amplification in mammary epithelial cells through cyclin D1/Cdk4 and Nek2." in: Oncogene, Vol. 29, Issue 36, pp. 5103-12, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).