A Kinase (PRKA) Anchor Protein 10 (AKAP10) (AA 1-101) antibody

Details for Product No. ABIN393884
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Antigen
Synonyms MGC84612, AKAP10, fc10g11, wu:fc10g11, si:dkey-197m14.4, AKAP-10, D-AKAP-2, D-AKAP2, PRKA10, 1500031L16Rik, B130049N18Rik, D-akap2, Akap10
Epitope
AA 1-101
(4), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity
Human
(36), (20), (19), (2), (2)
Host
Mouse
(28), (9), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(34), (21), (7), (4), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393884
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Immunogen AKAP10 (NP_009133, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 8C10
Isotype IgG2a kappa
Specificity AKAP10 (NP_009133, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP10
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 73818 DA
Gene ID 12406
NCBI Accession NP_009133
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA, therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-A Kinase (PRKA) Anchor Protein 10 (AKAP10) (AA 1-101) antibody Western blot analysis of AKAP10 over-expressed 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of AKAP10 expression in transfected 293T cell line by AKAP10 monoclonal antibody (M04), clone 8C10. Lane 1: AKAP10 transfected lysate(73.8 KDa). Lane 2: Non-transfected lysate.
anti-A Kinase (PRKA) Anchor Protein 10 (AKAP10) (AA 1-101) antibody (2) anti-A Kinase (PRKA) Anchor Protein 10 (AKAP10) (AA 1-101) antibody (Image 2)
Background publications Yoshida, Kato, Yokoi et al.: "Association of genetic variants with chronic kidney disease in individuals with different lipid profiles." in: International journal of molecular medicine, Vol. 24, Issue 2, pp. 233-46, 2009 (PubMed).

Eggers, Schafer, Goldenring et al.: "D-AKAP2 interacts with Rab4 and Rab11 through its RGS domains and regulates transferrin receptor recycling." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32869-80, 2009 (PubMed).

Zukowski, Bohatyrewicz, Biernawska et al.: "Association of the A1936G (rs203462) of A-kinase anchoring protein 10 polymorphisms with QT interval prolongation during kidney transplantation." in: Transplantation proceedings, Vol. 41, Issue 8, pp. 3036-8, 2009 (PubMed).

Sarma, Kinderman, Kim et al.: "Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity." in: Structure (London, England : 1993), Vol. 18, Issue 2, pp. 155-66, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Validation Images
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