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p65 antibody (Nuclear Factor-KB P65) (AA 432-506)

Details for Product anti-NFkBP65 Antibody No. ABIN393887, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
AA 432-506
103
77
67
53
49
47
44
42
40
37
33
25
22
16
15
15
14
13
13
10
10
9
9
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8
6
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1018
473
391
44
37
26
12
10
6
4
4
3
2
2
2
1
1
1
1
1
Host
Mouse
946
78
13
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This p65 antibody is un-conjugated
31
30
22
19
16
16
11
9
9
8
8
8
8
8
8
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
740
393
331
162
130
127
90
33
30
21
21
17
10
9
7
7
6
4
2
2
1
1
Supplier
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Immunogen RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 8G3
Isotype IgG1 lambda
Specificity RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification Purified
Alternative Name RELA (NFkBP65 Antibody Abstract)
Background NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: MGC131774, NFKB3v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)
Molecular Weight 60219 DA
Gene ID 5970
NCBI Accession NP_068810
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot = 1:500-1000
Comment

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer Phosphate buffered saline, pH 7.2
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (AA 432-506) (ABIN393887) Immunofluorescence of monoclonal antibody to RELA on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-p65 antibody (Nuclear Factor-KB P65) (AA 432-506) (ABIN393887) anti-Nuclear Factor-KB P65 (NFkBP65) (AA 432-506) antibody (Image 2)
Background publications Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Zhang, Li, Gao et al.: "Involvement of the p65/RelA subunit of NF-kappaB in TNF-alpha-induced SIRT1 expression in vascular smooth muscle cells." in: Biochemical and biophysical research communications, Vol. 397, Issue 3, pp. 569-75, 2010 (PubMed).

Tromp, Tonino, Elias et al.: "Dichotomy in NF-kappaB signaling and chemoresistance in immunoglobulin variable heavy-chain-mutated versus unmutated CLL cells upon CD40/TLR9 triggering." in: Oncogene, Vol. 29, Issue 36, pp. 5071-82, 2010 (PubMed).

Annunziata, Stavnes, Kleinberg et al.: "Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer." in: Cancer, Vol. 116, Issue 13, pp. 3276-84, 2010 (PubMed).

Jutooru, Chadalapaka, Lei et al.: "Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25332-44, 2010 (PubMed).

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