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Details for Product No. ABIN393887

Nuclear Factor-KB P65 (NFkBP65) antibody

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Antigen
Synonyms C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, zgc:100833, p65, NFkB
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393887
Quantity 0.05 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 8G3
Isotype IgG1 lambda
Specificity RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification Purified
Alternative Name RELA
Background NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: MGC131774, NFKB3v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)
Molecular Weight 60219 DA
Gene ID 5970
UniProt NP_068810
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence
Comment

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer Phosphate buffered saline, pH 7.2
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Jutooru, Chadalapaka, Lei et al.: "Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25332-44, 2010 (PubMed).

Annunziata, Stavnes, Kleinberg et al.: "Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer." in: Cancer, Vol. 116, Issue 13, pp. 3276-84, 2010 (PubMed).

Tromp, Tonino, Elias et al.: "Dichotomy in NF-kappaB signaling and chemoresistance in immunoglobulin variable heavy-chain-mutated versus unmutated CLL cells upon CD40/TLR9 triggering." in: Oncogene, Vol. 29, Issue 36, pp. 5071-82, 2010 (PubMed).

Zhang, Li, Gao et al.: "Involvement of the p65/RelA subunit of NF-kappaB in TNF-alpha-induced SIRT1 expression in vascular smooth muscle cells." in: Biochemical and biophysical research communications, Vol. 397, Issue 3, pp. 569-75, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Alternatives for antigen "Nuclear Factor-KB P65 (NFkBP65)", type "Antibodies"
Hosts (692), (53), (6), (4), (1)
Reactivities (734), (426), (348), (81), (78), (78), (38), (8), (6), (3), (2), (2), (2), (2), (2), (1)
Applications (553), (266), (248), (189), (111), (103), (100), (31), (17), (14), (13), (7), (5), (4), (3), (2), (2), (1)
Conjugates (16), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (2), (1), (1)
Epitopes (47), (43), (43), (42), (31), (31), (29), (28), (27), (22), (18), (15), (12), (7), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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