V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA) antibody

Antigen: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b

Clonality: Monoclonal (8G3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393887

Additional Information

Alternative name: RELA

Gene ID: 5970

UniProt: NP_068810

Immunogen: RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 lambda

Clone: 8G3

Description: Other names: MGC131774, NFKB3v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RELA (NP_068810, 432 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 60219 DA

Comments:

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to RELA (AT3616a) on HeLa cell . [antibody concentration 35 ug/ml] RELA monoclonal antibody (M01), clone 8G3 Western Blot analysis of RELA expression in HeLa NE ( Cat # L013V3 ).

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA) antibody (Image 2)

Publications

Product: Jutooru, Chadalapaka, Lei et al.: "Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25332-44, 2010 (PubMed).

Annunziata, Stavnes, Kleinberg et al.: "Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer." in: Cancer, Vol. 116, Issue 13, pp. 3276-84, 2010 (PubMed).

Tromp, Tonino, Elias et al.: "Dichotomy in NF-kappaB signaling and chemoresistance in immunoglobulin variable heavy-chain-mutated versus unmutated CLL cells upon CD40/TLR9 triggering." in: Oncogene, Vol. 29, Issue 36, pp. 5071-82, 2010 (PubMed).

Zhang, Li, Gao et al.: "Involvement of the p65/RelA subunit of NF-kappaB in TNF-alpha-induced SIRT1 expression in vascular smooth muscle cells." in: Biochemical and biophysical research communications, Vol. 397, Issue 3, pp. 569-75, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).