Adenylate Kinase 1 (AK1) (AA 1-195) antibody

Details for Product No. ABIN393890
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Antigen
Synonyms ADK-1, AK1, CG17146, DAK1, Dak1, Dmel\\CG17146, adk1, bs34e10.y1, ak5, ADENYLATE KINASE 1, MLE2.3, MLE2_3, adenylate kinase 1, Ak-1, B430205N08Rik, Ak 1, zgc:91930, Myokinase
Epitope
AA 1-195
(13), (8), (7), (3), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(93), (31), (29), (22), (14), (14), (14), (12), (5), (3), (2), (1), (1)
Host
Mouse
(72), (30), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(86), (47), (36), (19), (13), (11), (10), (5), (5), (5), (4), (2), (2), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393890
450.00 $
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Immunogen AK1 (AAH01116, 1 a.a. ~ 195 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone M1
Isotype IgG1 kappa
Specificity AK1 (AAH01116, 1 a.a. ~ 195 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AK1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 21635 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Adenylate Kinase 1 (AK1) (AA 1-195) antibody Immunofluorescence of monoclonal antibody to AK1 on HeLa cell (antibody concentration 20 µg/mL). AK1 monoclonal antibody (M08), clone M1 Western Blot analysis of AK1 expression in HeLa.
anti-Adenylate Kinase 1 (AK1) (AA 1-195) antibody (2) anti-Adenylate Kinase 1 (AK1) (AA 1-195) antibody (Image 2)
Background publications Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Topalis, Alvarez, Barral et al.: "Acyclic phosphonate nucleotides and human adenylate kinases: impact of a borano group on alpha-P position." in: Nucleosides, nucleotides & nucleic acids, Vol. 27, Issue 4, pp. 319-31, 2008 (PubMed).

Gloria-Bottini, Magrini, Pietropolli et al.: "Smoking and human reproduction: the effect of adenylate kinase genetic polymorphism." in: American journal of perinatology, Vol. 26, Issue 2, pp. 117-21, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia." in: European archives of psychiatry and clinical neuroscience, Vol. 259, Issue 3, pp. 151-63, 2009 (PubMed).

Validation Images
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