Breast Cancer Metastasis Suppressor 1 (BRMS1) antibody

Antigen: Breast Cancer Metastasis Suppressor 1 (BRMS1)

Synonyms: DKFZp564A063, MGC95128, MGC98869, BRMS1L, BRMS1

Clonality: Monoclonal (M1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393891

Additional Information

Alternative name: BRMS1

Gene ID: 25855

Immunogen: BRMS1 (AAH09834, 1 a.a. ~ 247 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: M1

Description: Other names: DKFZp564A063 breast cancer metastasis suppressor 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BRMS1 (AAH09834, 1 a.a. ~ 247 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 28461 DA

Comments:

Background: This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged BRMS1 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Cicek, Fukuyama, Cicek et al.: "BRMS1 contributes to the negative regulation of uPA gene expression through recruitment of HDAC1 to the NF-kappaB binding site of the uPA promoter." in: Clinical & experimental metastasis, Vol. 26, Issue 3, pp. 229-37, 2009 (PubMed).

Vaidya, Sanchez, Kim et al.: "Expression of the Breast Cancer Metastasis Suppressor 1 (BRMS1) maintains in vitro chemosensitivity of breast cancer cells." in: Cancer letters, Vol. 281, Issue 1, pp. 100-7, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation." in: BMC psychiatry, Vol. 9, pp. 17, 2009 (PubMed).

Frolova, Edmonds, Bodenstine et al.: "A shift from nuclear to cytoplasmic breast cancer metastasis suppressor 1 expression is associated with highly proliferative estrogen receptor-negative breast cancers." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 30, Issue 3, pp. 148-59, 2009 (PubMed).

Wu, McEwen, Harihar et al.: "BRMS1 expression alters the ultrastructural, biomechanical and biochemical properties of MDA-MB-435 human breast carcinoma cells: an AFM and Raman microspectroscopy study." in: Cancer letters, Vol. 293, Issue 1, pp. 82-91, 2010 (PubMed).