Charged Multivesicular Body Protein 2B (CHMP2B) antibody

Antigen: Charged Multivesicular Body Protein 2B (CHMP2B)

Synonyms: DMT1, VPS2B, VPS2-2, CHMP2.5, DKFZp564O123, 1190006E07Rik, RGD1306781, chmp2b, MGC77025, CHMP2B, LOC569532, MGC128858, DKFZp468C162

Clonality: Monoclonal (M1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393892

Additional Information

Alternative name: CHMP2B

Gene ID: 25978

Immunogen: CHMP2B (AAH01553, 1 a.a. ~ 592 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: M1

Description: Other names: CHMP2.5, DKFZP564O123 chromatin modifying protein 2B

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CHMP2B (AAH01553, 1 a.a. ~ 592 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 118544 DA

Comments:

Background: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged CHMP2B is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Cox, Ferraiuolo, Goodall et al.: "Mutations in CHMP2B in lower motor neuron predominant amyotrophic lateral sclerosis (ALS)." in: PLoS ONE, Vol. 5, Issue 3, pp. e9872, 2010 (PubMed).

Kaivorinne, Krüger, Udd et al.: "Mutations in CHMP2B are not a cause of frontotemporal lobar degeneration in Finnish patients." in: European journal of neurology : the official journal of the European Federation of Neurological Societies, Vol. 17, Issue 11, pp. 1393-5, 2010 (PubMed).

Yamazaki, Takahashi, Hiji et al.: "Immunopositivity for ESCRT-III subunit CHMP2B in granulovacuolar degeneration of neurons in the Alzheimer's disease hippocampus." in: Neuroscience letters, Vol. 477, Issue 2, pp. 86-90, 2010 (PubMed).

Tsai, Soong, Lin et al.: "FUS, TARDBP, and SOD1 mutations in a Taiwanese cohort with familial ALS." in: Neurobiology of aging, Vol. 32, Issue 3, pp. 553.e13-21, 2011 (PubMed).

Ghanim, Guillot-Noel, Pasquier et al.: "CHMP2B mutations are rare in French families with frontotemporal lobar degeneration." in: Journal of neurology, Vol. 257, Issue 12, pp. 2032-6, 2010 (PubMed).