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Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (AA 151-251) antibody

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Synonyms nf45, pro3063, ILF2, wu:fb34h01, zgc:73084, zgc:77276, NF45, PRO3063, 6230405A16Rik, TEG-267, Tex261, Tex267
AA 151-251
(18), (14), (13), (10), (4), (4), (2), (2), (1)
(88), (15), (13), (8), (4), (4), (3), (3), (3), (3), (1), (1)
(58), (20), (11)
Clonality (Clone)
Monoclonal ()
(8), (8), (8), (3), (3), (3)
ELISA, Western Blotting (WB)
(78), (53), (28), (16), (9), (4), (2), (2)
Pubmed 5 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 8 to 11 Business Days
Immunogen ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E2
Isotype IgG1 kappa
Specificity ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ILF2 (ILF2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 43062 DA
Gene ID 12406
NCBI Accession NP_004506
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (AA 151-251) antibody (ABIN393897) Detection limit for recombinant GST tagged ILF2 is approximately 1 ng/mL as a capture...
Background publications Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Kiesler, Haynes, Shi et al.: "NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 8256-67, 2010 (PubMed).

Graber, Baird, Kao et al.: "NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response." in: Cell death and differentiation, Vol. 17, Issue 4, pp. 719-29, 2010 (PubMed).

Sakamoto, Aoki, Higuchi et al.: "The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3754-69, 2009 (PubMed).

Guan, Altan-Bonnet, Parrott et al.: "Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control." in: Molecular and cellular biology, Vol. 28, Issue 14, pp. 4629-41, 2008 (PubMed).

Catalog No. ABIN393897
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