Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) antibody

Details for Product No. ABIN393897
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Synonyms NF45, PRO3063, nf45, Tex261, Tex267, TEG-267, 6230405A16Rik, zgc:73084, zgc:77276, wu:fb34h01, pro3063, ILF2
(28), (6), (5), (5), (1), (1), (1)
(18), (7), (6)
Clonality (Clone)
Monoclonal ()
(2), (2), (2)
ELISA, Western Blotting (WB)
(29), (17), (7), (6), (6), (2), (2)
Pubmed 5 references available
Catalog no. ABIN393897
Quantity 0.1 mg
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Immunogen ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E2
Isotype IgG1 kappa
Specificity ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ILF2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 43062 DA
Gene ID 12406
UniProt NP_004506
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) antibody Detection limit for recombinant GST tagged ILF2 is approximately 1 ng/mL as a capture antibody.
Background publications Guan, Altan-Bonnet, Parrott et al.: "Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control." in: Molecular and cellular biology, Vol. 28, Issue 14, pp. 4629-41, 2008 (PubMed).

Sakamoto, Aoki, Higuchi et al.: "The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3754-69, 2009 (PubMed).

Graber, Baird, Kao et al.: "NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response." in: Cell death and differentiation, Vol. 17, Issue 4, pp. 719-29, 2010 (PubMed).

Kiesler, Haynes, Shi et al.: "NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 8256-67, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Hosts (18), (7), (6)
Reactivities (28), (6), (5), (5), (1), (1), (1)
Applications (29), (17), (7), (6), (6), (2), (2)
Conjugates (2), (2), (2)
Epitopes (5), (5), (4), (3), (2), (2)
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