Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) antibody

Antigen: Interleukin Enhancer Binding Factor 2, 45kDa (ILF2)

Synonyms: NF45, MGC8391, PRO3063, ilf2, MGC53292, nf45, pro3063, MGC75701, ILF2, MGC134524, DKFZp469J1839

Clonality: Monoclonal (1E2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393897

Additional Information

Alternative name: ILF2

Gene ID: 3608

UniProt: NP_004506

Immunogen: ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1E2

Description: Other names: MGC8391, NF45, PRO3063 interleukin enhancer binding factor 2, 45kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ILF2 (NP_004506, 151 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 43062 DA

Comments:

Background: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ILF2 is approximately 1ng/ml as a capture antibody.

Publications

Product: Guan, Altan-Bonnet, Parrott et al.: "Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control." in: Molecular and cellular biology, Vol. 28, Issue 14, pp. 4629-41, 2008 (PubMed).

Sakamoto, Aoki, Higuchi et al.: "The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3754-69, 2009 (PubMed).

Graber, Baird, Kao et al.: "NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response." in: Cell death and differentiation, Vol. 17, Issue 4, pp. 719-29, 2010 (PubMed).

Kiesler, Haynes, Shi et al.: "NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 8256-67, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).