Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) (AA 81-191) antibody

Details for Product No. ABIN393901
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Antigen
Synonyms CG6755, Dmel\\CG6755, Elo-A, dEloA, tceb3a, zgc:56638, wu:fw66g10, TCEB3, 110kDa, AA408125, Tceb3a, El, EloA, SIII, SIII p110, TCEB3A
Epitope
AA 81-191
(12), (9), (7), (6), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(60), (20), (18), (13), (12), (1)
Host
Mouse
(52), (6), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(48), (23), (10), (10), (7), (6), (5), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393901
450.00 $
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Immunogen TCEB3 (NP_003189, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E2
Isotype IgG2a kappa
Specificity TCEB3 (NP_003189, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TCEB3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 87230 DA
Gene ID 12406
NCBI Accession NP_003189
Research Area Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) (AA 81-191) antibody Western Blot analysis of TCEB3 expression in transfected 293T cell line by TCEB3 monoclonal antibody (M01), clone 3E2. Lane 1: TCEB3 transfected lysate(87.2 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged TCEB3 is approximately 0.1 ng/mL as a capture antibody.
anti-Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) (AA 81-191) antibody (2) anti-Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) (AA 81-191) antibody (Image 2)
Background publications Woo, Imm, Min et al.: "Structural and functional insights into the B30.2/SPRY domain." in: The EMBO journal, Vol. 25, Issue 6, pp. 1353-63, 2006 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Landa, Ruiz-Llorente, Montero-Conde et al.: "The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors." in: PLoS genetics, Vol. 5, Issue 9, pp. e1000637, 2009 (PubMed).

Validation Images
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