RNA Binding Motif, Single Stranded Interacting Protein 2 (RBMS2) antibody

Antigen: RNA Binding Motif, Single Stranded Interacting Protein 2 (RBMS2)

Synonyms: SCR3, FLJ39093, FLJ40023, FLJ43262, Scr3, 2610315E04Rik, RBMS2, MGC128164

Clonality: Monoclonal (4E2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393904

Additional Information

Alternative name: RBMS2

Gene ID: 5939

UniProt: NP_002889

Immunogen: RBMS2 (NP_002889, 308 a.a. ~ 407 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4E2

Description: Other names: SCR3 RNA binding motif, single stranded interacting protein 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RBMS2 (NP_002889, 308 a.a. ~ 407 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 43959 DA

Comments:

Background: The protein encoded by this gene is a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. The RBMS proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. This protein was isolated by phenotypic complementation of cdc2 and cdc13 mutants of yeast and is thought to suppress cdc2 and cdc13 mutants through the induction of translation of cdc2.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged RBMS2 is approximately 1ng/ml as a capture antibody.

Publications

Product: Bonaldo, Lennon, Soares: "Normalization and subtraction: two approaches to facilitate gene discovery." in: Genome research, Vol. 6, Issue 9, pp. 791-806, 1997 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).