Interferon, gamma-Inducible Protein 16 (IFI16) antibody

Antigen: Interferon, gamma-Inducible Protein 16 (IFI16)

Synonyms: PYHIN2, IFNGIP1, MGC9466, p204, Ifi16, IFI16, DKFZp468E1111

Clonality: Monoclonal (2E3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393907

Additional Information

Alternative name: IFI16

Gene ID: 3428

Immunogen: IFI16 (AAH17059, 630 a.a. ~ 730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2E3

Description: Other names: IFNGIP1, PYHIN2 interferon, gamma-inducible protein 16

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: IFI16 (AAH17059, 630 a.a. ~ 730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 88256 DA

Comments:

Background: This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to IFI16 (AT2484a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of IFI16 expression in transfected 293T cell line by IFI16 monoclonal antibody (M03), clone 2E3. Lane 1: IFI16 transfected lysate(82 KDa). Lane 2: Non-transfected lysate.

anti-Interferon, gamma-Inducible Protein 16 (IFI16) antibody (Image 2)

Publications

Product: Yu, Hao, Zhao et al.: "Delta-like 1 contributes to cell growth by increasing the interferon-inducible protein 16 expression in hepatocellular carcinoma." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 30, Issue 5, pp. 703-14, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Cristea, Moorman, Terhune et al.: "Human cytomegalovirus pUL83 stimulates activity of the viral immediate-early promoter through its interaction with the cellular IFI16 protein." in: Journal of virology, Vol. 84, Issue 15, pp. 7803-14, 2010 (PubMed).

Kimkong, Avihingsanon, Hirankarn: "Association of IFI200 gene polymorphisms with susceptibility to systemic lupus erythematosus." in: The Journal of rheumatology, Vol. 37, Issue 7, pp. 1544-7, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).