|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: MGC26254 TFIIA-alpha/beta-like factor|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||52445 DA|
Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|Western Blot analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4. Lane 1: ALF transfected lysate(52.4 KDa). Lane 2: Non-transfected lysate.|
Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).
Huang, Wang, Li et al.: "Involvement of ALF in human spermatogenesis and male infertility." in: International journal of molecular medicine, Vol. 17, Issue 4, pp. 599-604, 2006 (PubMed).
Howe, Mehmud, Saha et al.: "Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo." in: Stem cells and development, Vol. 15, Issue 2, pp. 175-90, 2006 (PubMed).
Kim, Li, Cui et al.: "Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34288-98, 2006 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).