Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody

Antigen: Hepatocyte Specific Developmental Regulation 1 (HSDR1)

Clonality: Monoclonal (3E4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393912

Additional Information

Alternative name: ALF

Gene ID: 11036

UniProt: NP_006863

Immunogen: ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3E4

Description: Other names: MGC26254 TFIIA-alpha/beta-like factor

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 52445 DA

Comments:

Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4. Lane 1: ALF transfected lysate(52.4 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Huang, Wang, Li et al.: "Involvement of ALF in human spermatogenesis and male infertility." in: International journal of molecular medicine, Vol. 17, Issue 4, pp. 599-604, 2006 (PubMed).

Howe, Mehmud, Saha et al.: "Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo." in: Stem cells and development, Vol. 15, Issue 2, pp. 175-90, 2006 (PubMed).

Kim, Li, Cui et al.: "Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34288-98, 2006 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).