Kruppel-Like Factor 12 (KLF12) antibody

Antigen: Kruppel-Like Factor 12 (KLF12)

Synonyms: AP2REP, AP-2rep, HSPC122, AI225908, AI315654, 2700063E05Rik, B130052C06Rik, D530033K05Rik, klf12, zgc:171689, KLF12

Clonality: Monoclonal (3E4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393913

Additional Information

Alternative name: KLF12

Gene ID: 11278

UniProt: NP_009180

Immunogen: KLF12 (NP_009180, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3E4

Description: Other names: AP-2rep, AP2REP, HSPC122 Kruppel-like factor 12

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: KLF12 (NP_009180, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 44240 DA

Comments:

Background: Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of KLF12 expression in transfected 293T cell line by KLF12 monoclonal antibody (M01), clone 3E4. Lane 1: KLF12 transfected lysate(44.2 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Nakamura, Migita, Hosoda et al.: "Krüppel-like factor 12 plays a significant role in poorly differentiated gastric cancer progression." in: International journal of cancer. Journal international du cancer, Vol. 125, Issue 8, pp. 1859-67, 2009 (PubMed).

Eyre, Flynn, Martin et al.: "No evidence for association of the KLF12 gene with rheumatoid arthritis in a large UK cohort." in: Annals of the rheumatic diseases, Vol. 69, Issue 7, pp. 1407-8, 2010 (PubMed).

Wieczorek, Holle, Müller et al.: "A functionally relevant IRF5 haplotype is associated with reduced risk to Wegener's granulomatosis." in: Journal of molecular medicine (Berlin, Germany), Vol. 88, Issue 4, pp. 413-21, 2010 (PubMed).

Petersen, Amundadottir, Fuchs et al.: "A genome-wide association study identifies pancreatic cancer susceptibility loci on chromosomes 13q22.1, 1q32.1 and 5p15.33." in: Nature genetics, Vol. 42, Issue 3, pp. 224-8, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).