TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C) (AA 761-870) antibody

Details for Product No. ABIN393929
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Antigen
Synonyms TAF1C, Tafi95, mTAFI95, MGC:39976, SL1, TAFI110, TAFI95
Epitope
AA 761-870
(4), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(13), (3), (1), (1), (1)
Host
Mouse
(12), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(13), (3), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393929
450.00 $
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Immunogen TAF1C (NP_005670, 761 a.a. ~ 870 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E6
Isotype IgG2a kappa
Specificity TAF1C (NP_005670, 761 a.a. ~ 870 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF1C
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 95274 DA
Gene ID 12406
NCBI Accession NP_005670
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C) (AA 761-870) antibody Western Blot analysis of TAF1C expression in transfected 293T cell line by TAF1C monoclonal antibody (M02), clone 3E6. Lane 1: TAF1C transfected lysate(85.1 KDa). Lane 2: Non-transfected lysate.
Background publications Friedrich, Panov, Cabart et al.: "TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter." in: The Journal of biological chemistry, Vol. 280, Issue 33, pp. 29551-8, 2005 (PubMed).

Zhang, Comai, Johnson: "PTEN represses RNA Polymerase I transcription by disrupting the SL1 complex." in: Molecular and cellular biology, Vol. 25, Issue 16, pp. 6899-911, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Anney, Klei, Pinto et al.: "A genome-wide scan for common alleles affecting risk for autism." in: Human molecular genetics, Vol. 19, Issue 20, pp. 4072-82, 2010 (PubMed).

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