TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C) antibody

Antigen: TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C)

Synonyms: SL1, TAFI95, TAFI110, MGC:39976, Tafi95, mTAFI95, TAF1C, MGC139975

Clonality: Monoclonal (3E6)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393929

Additional Information

Alternative name: TAF1C

Gene ID: 9013

UniProt: NP_005670

Immunogen: TAF1C (NP_005670, 761 a.a. ~ 870 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3E6

Description: Other names: MGC:39976, SL1, TAFI110, TAFI95 TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TAF1C (NP_005670, 761 a.a. ~ 870 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 95274 DA

Comments:

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of TAF1C expression in transfected 293T cell line by TAF1C monoclonal antibody (M02), clone 3E6. Lane 1: TAF1C transfected lysate(85.1 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Friedrich, Panov, Cabart et al.: "TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter." in: The Journal of biological chemistry, Vol. 280, Issue 33, pp. 29551-8, 2005 (PubMed).

Zhang, Comai, Johnson: "PTEN represses RNA Polymerase I transcription by disrupting the SL1 complex." in: Molecular and cellular biology, Vol. 25, Issue 16, pp. 6899-911, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Anney, Klei, Pinto et al.: "A genome-wide scan for common alleles affecting risk for autism." in: Human molecular genetics, Vol. 19, Issue 20, pp. 4072-82, 2010 (PubMed).