Forkhead Box A2 (FOXA2) antibody

Antigen: Forkhead Box A2 (FOXA2)

Synonyms: HNF3B, TCF3B, MGC19807, Hnf3b, Tcf3b, Hnf-3b, Tcf-3b, HNF3beta, HNF3-beta, ax, axl, fkd1, HNF-3B, MGC101552, tdsubc_2g8, wu:fe05g04, ik:tdsubc_2g8, xx:tdsubc_2g8, hnf3b, tcf3b, hnf3beta, hnf3-beta, FOXA2, HNF-3BETA, HNF-3b, xFD-3, FoxA2a, FoxA2-A, xbeta-1

Clonality: Monoclonal (7E6)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393931

Additional Information

Alternative name: FOXA2

Gene ID: 3170

UniProt: NP_068556

Immunogen: FOXA2 (NP_068556, 363 a.a. ~ 458 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 7E6

Description: Other names: HNF3B, MGC19807, TCF3Bforkhead box A2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FOXA2 (NP_068556, 363 a.a. ~ 458 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 48306 DA

Comments:

Background: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.

Synonyms: HNF3B, TCF3B, MGC19807, Hnf3b, Tcf3b, Hnf-3b, Tcf-3b, HNF3beta, HNF3-beta, ax, axl, fkd1, HNF-3B, MGC101552, tdsubc_2g8, wu:fe05g04, ik:tdsubc_2g8, xx:tdsubc_2g8, hnf3b, tcf3b, hnf3beta, hnf3-beta, FOXA2, HNF-3BETA, HNF-3b, xFD-3, FoxA2a, FoxA2-A, xbeta-1

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to FOXA2 (AT2085a) on HepG2 cell . [antibody concentration 10 ug/ml] Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi ( Cat # H00003170-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody (M01), clone 7E6 (Cat # AT2085a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Forkhead Box A2 (FOXA2) antibody (Image 2)

Publications

Product: Xing, Cohen, Boerwinkle: "A weighted false discovery rate control procedure reveals alleles at FOXA2 that influence fasting glucose levels." in: American journal of human genetics, Vol. 86, Issue 3, pp. 440-6, 2010 (PubMed).

Song, Washington, Crawford: "Loss of FOXA1/2 is essential for the epithelial-to-mesenchymal transition in pancreatic cancer." in: Cancer research, Vol. 70, Issue 5, pp. 2115-25, 2010 (PubMed).

Banerjee, Meyer, Mazumdar et al.: "Hepatitis C virus differentially modulates activation of forkhead transcription factors and insulin-induced metabolic gene expression." in: Journal of virology, Vol. 84, Issue 12, pp. 5936-46, 2010 (PubMed).

Dayoub, Groitl, Dobner et al.: "Foxa2 (HNF-3beta) regulates expression of hepatotrophic factor ALR in liver cells." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 465-70, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).