Inhibitor of Growth Family, Member 3 (ING3) antibody

Antigen: Inhibitor of Growth Family, Member 3 (ING3)

Synonyms: Eaf4, ING2, MEAF4, p47ING3, FLJ20089, MGC112729, ing3, MGC52705, MGC56327, zgc:56327, ING3, DKFZp459N2251, P47ING3, 1300013A07Rik

Clonality: Monoclonal (2E9)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393949

Additional Information

Alternative name: ING3

Gene ID: 54556

UniProt: NP_938008

Immunogen: ING3 (NP_938008, 1 a.a. ~ 93 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E9

Description: Other names: Eaf4, FLJ20089, ING2, p47ING3 inhibitor of growth family, member 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ING3 (NP_938008, 1 a.a. ~ 93 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 46743 DA

Comments:

Background: The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

ING3 monoclonal antibody (M01), clone 2E9. Western Blot analysis of ING3 expression in Hela NE ( Cat # L013V3 ).

Publications

Product: Shi, Hong, Walter et al.: "ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression." in: Nature, Vol. 442, Issue 7098, pp. 96-9, 2006 (PubMed).

Wang, Dai, Martinka et al.: "Prognostic significance of nuclear ING3 expression in human cutaneous melanoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 14, pp. 4111-6, 2007 (PubMed).

Gunduz, Beder, Gunduz et al.: "Downregulation of ING3 mRNA expression predicts poor prognosis in head and neck cancer." in: Cancer science, Vol. 99, Issue 3, pp. 531-8, 2008 (PubMed).

Maestrini, Pagnamenta, Lamb et al.: "High-density SNP association study and copy number variation analysis of the AUTS1 and AUTS5 loci implicate the IMMP2L-DOCK4 gene region in autism susceptibility." in: Molecular psychiatry, Vol. 15, Issue 9, pp. 954-68, 2010 (PubMed).

Chen, Wang, Garate et al.: "The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system." in: Oncogene, Vol. 29, Issue 10, pp. 1498-508, 2010 (PubMed).