Did you know that you can buy products from over 140 different suppliers from us?

TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (Met11) antibody

Details for Product No. ABIN393958
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
TAF2J, TAFII20, TFIID, taf12-a, CG17358, Dmel\\CG17358, T2DA_DROME, TAF, TAF12, TAF30a, TAF30alpha, TAF30alpha/28, TAF30alpha/28/22, TAFII30alpha/22, TAF[II]30alpha, TAF[[II]], TAF[[II]]230, TAF[[II]] ... show more
TAF2J, TAFII20, TFIID, taf12-a, CG17358, Dmel\\CG17358, T2DA_DROME, TAF, TAF12, TAF30a, TAF30alpha, TAF30alpha/28, TAF30alpha/28/22, TAFII30alpha/22, TAF[II]30alpha, TAF[[II]], TAF[[II]]230, TAF[[II]]30alpha, TAF[[II]]30alpha/28 TAF[[II]]22, TAF[[II]]30alpha/28/22, TFIID 22, TFIID 28, TFIID-p28, Taf22, Taf28, Taf30alpha, Taf32, Taf[[II]]30alpha, d28/22, dTAF12, dTAF22, dTAF28, dTAFII30, dTAFII30 alpha, dTAF[[II]]30, dTAF[[II]]30alpha, dTAF[[II]]30alpha/22, dmTAF12, p22, p28 show less
Epitope
Met11
(3), (2), (2), (1)
Reactivity
Human
(26), (19), (17), (13), (13), (13), (12)
Host
Mouse
(19), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(18), (11), (10), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN393958
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen TAF12 (AAH11986, 1 a.a. ~ 162 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E10
Isotype IgG2a kappa
Specificity TAF12 (AAH11986, 1 a.a. ~ 162 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF12
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17924 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (Met11) antibody TAF12 monoclonal antibody (M11), clone 1E10. Western Blot analysis of TAF12 expression in Hela NE.
Background publications Helmlinger, Hardy, Abou-Sleymane et al.: "Glutamine-expanded ataxin-7 alters TFTC/STAGA recruitment and chromatin structure leading to photoreceptor dysfunction." in: PLoS biology, Vol. 4, Issue 3, pp. e67, 2006 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Denissov, van Driel, Voit et al.: "Identification of novel functional TBP-binding sites and general factor repertoires." in: The EMBO journal, Vol. 26, Issue 4, pp. 944-54, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Gazit, Moshonov, Elfakess et al.: "TAF4/4b x TAF12 displays a unique mode of DNA binding and is required for core promoter function of a subset of genes." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26286-96, 2009 (PubMed).

Hosts (19), (8)
Reactivities (26), (19), (17), (13), (13), (13), (12)
Applications (18), (11), (10), (4), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN393958
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top