RAN Binding Protein 3 (RANBP3) antibody

Antigen: RAN Binding Protein 3 (RANBP3)

Synonyms: DKFZp586I1520, AA408221, 2610024N24Rik, ranbp3, MGC79798, RANBP3, DKFZp459E0714

Clonality: Monoclonal (2E10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393959

Additional Information

Alternative name: RANBP3

Gene ID: 8498

Immunogen: RANBP3 (AAH04349, 298 a.a. ~ 397 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E10

Description: Other names: DKFZp586I1520 RAN binding protein 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RANBP3 (AAH04349, 298 a.a. ~ 397 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 60210 DA

Comments:

Background: This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged RANBP3 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Oh, Yang, Hahn et al.: "Transcriptome analysis of human gastric cancer." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 16, Issue 12, pp. 942-54, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Yoon, Shin, Liu et al.: "Ran-binding protein 3 phosphorylation links the Ras and PI3-kinase pathways to nucleocytoplasmic transport." in: Molecular cell, Vol. 29, Issue 3, pp. 362-75, 2008 (PubMed).

Dai, Lin, Chang et al.: "Nuclear export of Smad2 and Smad3 by RanBP3 facilitates termination of TGF-beta signaling." in: Developmental cell, Vol. 16, Issue 3, pp. 345-57, 2009 (PubMed).