AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) (AA 317-417) antibody

Details for Product No. ABIN393983
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Antigen
Synonyms ARID3A, dril1, dril3, bright, e2fbp1, BRIGHT, DRIL1, DRIL3, E2FBP1, Dril1, Bright, Dri1
Epitope
AA 317-417
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(38), (17), (17), (14), (12), (12), (2), (1)
Host
Mouse
(27), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(28), (13), (10), (10), (10), (4), (4), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393983
450.00 $
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Immunogen ARID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A11
Isotype IgG1 kappa
Specificity ARID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ARID3A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 62889 DA
Gene ID 12406
NCBI Accession NP_005215
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) (AA 317-417) antibody Immunofluorescence of to ARID3A on HeLa cell . Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi used as specificity and loading control.
anti-AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) (AA 317-417) antibody (2) anti-AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) (AA 317-417) antibody (Image 2)
Background publications Lin, Ippolito, Zong et al.: "Bright/ARID3A contributes to chromatin accessibility of the immunoglobulin heavy chain enhancer." in: Molecular cancer, Vol. 6, pp. 23, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lin, Zhou, Zheng et al.: "Cross talk between Id1 and its interactive protein Dril1 mediate fibroblast responses to transforming growth factor-beta in pulmonary fibrosis." in: The American journal of pathology, Vol. 173, Issue 2, pp. 337-46, 2008 (PubMed).

Schmidt, Kim, Ippolito et al.: "Signalling of the BCR is regulated by a lipid rafts-localised transcription factor, Bright." in: The EMBO journal, Vol. 28, Issue 6, pp. 711-24, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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