AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) antibody

Antigen: AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A)

Synonyms: arid3a, DRIL1, DRIL3, BRIGHT, E2FBP1, Dri1, Dril1, Bright, ARID3A, dril1, dril3, bright, e2fbp1

Clonality: Monoclonal (1A11)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393983

Additional Information

Alternative name: ARID3A

Gene ID: 1820

UniProt: NP_005215

Immunogen: ARID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1A11

Description: Other names: BRIGHT, DRIL1, DRIL3, E2FBP1AT rich interactive domain 3A (BRIGHT- like)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ARID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 62889 DA

Comments:

Background: This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of to ARID3A ( Cat# AT1191a ) on HeLa cell . Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi ( Cat # H00001820-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody (M01), clone 1A11 (Cat # AT1191a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A) antibody (Image 2)

Publications

Product: Lin, Ippolito, Zong et al.: "Bright/ARID3A contributes to chromatin accessibility of the immunoglobulin heavy chain enhancer." in: Molecular cancer, Vol. 6, pp. 23, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lin, Zhou, Zheng et al.: "Cross talk between Id1 and its interactive protein Dril1 mediate fibroblast responses to transforming growth factor-beta in pulmonary fibrosis." in: The American journal of pathology, Vol. 173, Issue 2, pp. 337-46, 2008 (PubMed).

Schmidt, Kim, Ippolito et al.: "Signalling of the BCR is regulated by a lipid rafts-localised transcription factor, Bright." in: The EMBO journal, Vol. 28, Issue 6, pp. 711-24, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).