ZFP36 Ring Finger Protein-Like 1 (ZFP36L1) antibody

Antigen: ZFP36 Ring Finger Protein-Like 1 (ZFP36L1)

Clonality: Monoclonal (1A3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393989

Additional Information

Alternative name: ZFP36L1

Gene ID: 677

UniProt: NP_004917

Immunogen: ZFP36L1 (NP_004917, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1A3

Description: Other names: BRF1, Berg36, ERF1, ERF1, RNF162B, TIS11B, cMG1 zinc finger protein 36, C3H type-like 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ZFP36L1 (NP_004917, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 36314 DA

Comments:

Background: This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ZFP36L1 over-expressed 293 cell line, cotransfected with ZFP36L1 Validated Chimera RNAi ( Cat # H00000677-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZFP36L1 monoclonal antibody (M02), clone 1A3 (Cat # AT4574a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of ZFP36L1 expression in transfected 293T cell line by ZFP36L1 monoclonal antibody (M02), clone 1A3. Lane 1: ZFP36L1 transfected lysate(36.3 KDa). Lane 2: Non-transfected lysate.

anti-ZFP36 Ring Finger Protein-Like 1 (ZFP36L1) antibody (Image 2)

Publications

Product: Baou, Jewell, Muthurania et al.: "Involvement of Tis11b, an AU-rich binding protein, in induction of apoptosis by rituximab in B cell chronic lymphocytic leukemia cells." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 23, Issue 5, pp. 986-9, 2009 (PubMed).

Cheng, Saito, Pisarev et al.: "Structural insights into eRF3 and stop codon recognition by eRF1." in: Genes & development, Vol. 23, Issue 9, pp. 1106-18, 2009 (PubMed).

Sinha, Dutta, Datta et al.: "Von Hippel-Lindau gene product modulates TIS11B expression in renal cell carcinoma: impact on vascular endothelial growth factor expression in hypoxia." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32610-8, 2009 (PubMed).

Hacker, Valchanova, Adams et al.: "ZFP36L1 is regulated by growth factors and cytokines in keratinocytes and influences their VEGF production." in: Growth factors (Chur, Switzerland), Vol. 28, Issue 3, pp. 178-90, 2010 (PubMed).

Dubois, Trynka, Franke et al.: "Multiple common variants for celiac disease influencing immune gene expression." in: Nature genetics, Vol. 42, Issue 4, pp. 295-302, 2010 (PubMed).