T-Complex Protein 1 Subunit zeta-2-Like (CCT6B) antibody

Antigen: T-Complex Protein 1 Subunit zeta-2-Like (CCT6B)

Clonality: Monoclonal (1A4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393990

Additional Information

Alternative name: CCT6B

Gene ID: 10693

Immunogen: CCT6B (AAH27591, 1 a.a. ~ 531 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1A4

Description: Other names: CCTzeta2, CCTZ2, Cctz2, TCP1zeta2, TSA303 chaperonin containing TCP1, subunit 6B (zeta 2)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CCT6B (AAH27591, 1 a.a. ~ 531 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 57765 DA

Comments:

Background: This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of CCT6B expression in transfected 293T cell line by CCT6B monoclonal antibody (M01), clone 1A4. Lane 1: CCT6B transfected lysate(57.8 KDa). Lane 2: Non-transfected lysate. CCT6B monoclonal antibody (M01), clone 1A4 Western Blot analysis of CCT6B expression in HL-60 ( Cat # L014V1 ).

anti-T-Complex Protein 1 Subunit zeta-2-Like (CCT6B) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Stirling, Cuéllar, Alfaro et al.: "PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7012-21, 2006 (PubMed).

Goudreault, DAmbrosio, Kean et al.: "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 1, pp. 157-71, 2009 (PubMed).

Mukherjee, Conway de Macario, Macario et al.: "Chaperonin genes on the rise: new divergent classes and intense duplication in human and other vertebrate genomes." in: BMC evolutionary biology, Vol. 10, pp. 64, 2010 (PubMed).