BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 1 (BACH1) antibody

Antigen: BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 1 (BACH1)

Synonyms: BACH-1, BACH1, MGC112205, zgc:112205

Clonality: Monoclonal (1B8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394012

Additional Information

Alternative name: BACH1

Gene ID: 571

UniProt: NP_996749

Immunogen: BACH1 (NP_996749, 396 a.a. ~ 493 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1B8

Description: Other names: BTB and CNC homology 1, basic leucine zipper transcription factor 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BACH1 (NP_996749, 396 a.a. ~ 493 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 81958 DA

Comments:

Background: This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

BACH1 monoclonal antibody (M02), clone 1B8 Western Blot analysis of BACH1 expression in NIH/3T3 ( Cat # L018V1 ). Detection limit for recombinant GST tagged BACH1 is approximately 0.03ng/ml as a capture antibody.

anti-BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 1 (BACH1) antibody (Image 2)

Publications

Product: Goven, Boutten, Leçon-Malas et al.: "Prolonged cigarette smoke exposure decreases heme oxygenase-1 and alters Nrf2 and Bach1 expression in human macrophages: roles of the MAP kinases ERK(1/2) and JNK." in: FEBS letters, Vol. 583, Issue 21, pp. 3508-18, 2009 (PubMed).

Zhong, Raval, Edwards et al.: "A role for Bach1 and HO-2 in suppression of basal and UVA-induced HO-1 expression in human keratinocytes." in: Free radical biology & medicine, Vol. 48, Issue 2, pp. 196-206, 2010 (PubMed).

Hou, Tian, Zheng et al.: "MicroRNA-196 represses Bach1 protein and hepatitis C virus gene expression in human hepatoma cells expressing hepatitis C viral proteins." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 5, pp. 1494-504, 2010 (PubMed).

Gong, Kim, Leung et al.: "BACH1/FANCJ acts with TopBP1 and participates early in DNA replication checkpoint control." in: Molecular cell, Vol. 37, Issue 3, pp. 438-46, 2010 (PubMed).

Miyazaki, Kirino, Takeno et al.: "Expression of heme oxygenase-1 in human leukemic cells and its regulation by transcriptional repressor Bach1." in: Cancer science, Vol. 101, Issue 6, pp. 1409-16, 2010 (PubMed).