Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody

Antigen: Non-SMC Condensin I Complex, Subunit H (NCAPH)

Synonyms: BRRN1, CAP-H, HCAP-H, NCAPH, MGC127159, MGC158618, si:dkeyp-86b9.4

Clonality: Monoclonal (1C9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394025

Additional Information

Alternative name: BRRN1

Gene ID: 23397

Immunogen: BRRN1 (AAH24211, 645 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1C9

Description: Other names: HCAPH, MGC4616barren homolog (Drosophila)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BRRN1 (AAH24211, 645 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 82563 DA

Comments:

Background: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

BRRN1 monoclonal antibody (M01), clone 1C9 Western Blot analysis of BRRN1 expression in Jurkat ( Cat # L017V1 ). Detection limit for recombinant GST tagged BRRN1 is approximately 0.03ng/ml as a capture antibody.

anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Heale, Ball, Schmiesing et al.: "Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair." in: Molecular cell, Vol. 21, Issue 6, pp. 837-48, 2006 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).