Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody

Details for Product No. ABIN394025
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Antigen
Synonyms BRRN1, CAP-H, A730011O11Rik, Brrn1, HCAP-H, mKIAA0074, brrn1, cap-h, NCAPH, si:dkeyp-86b9.4, zgc:158618
Reactivity
Human
(50), (16), (16), (13), (11), (1), (1)
Host
Mouse
(43), (5), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(40), (22), (9), (5), (4), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394025
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen BRRN1 (AAH24211, 645 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C9
Isotype IgG1 kappa
Specificity BRRN1 (AAH24211, 645 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BRRN1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 82563 DA
Gene ID 12406
Research Area Signaling, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody BRRN1 monoclonal antibody (M01), clone 1C9 Western Blot analysis of BRRN1 expression in Jurkat. Detection limit for recombinant GST tagged BRRN1 is approximately 0.03 ng/mL as a capture antibody.
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody (2) anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Heale, Ball, Schmiesing et al.: "Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair." in: Molecular cell, Vol. 21, Issue 6, pp. 837-48, 2006 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Hosts (43), (5), (5)
Reactivities (50), (16), (16), (13), (11), (1), (1)
Applications (40), (22), (9), (5), (4), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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