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PML antibody (Promyelocytic Leukemia) (AA 411-510)

Details for Product anti-PML Antibody No. ABIN394032, Supplier: Log in to see
Antigen
  • 1200009E24Rik
  • AI661194
  • MYL
  • PML
  • PP8675
  • RGD1562602
  • RNF71
  • TRIM19
  • Trim19
Alternatives
anti-Human PML antibody for Western Blotting
Epitope
AA 411-510
13
12
9
9
9
7
7
5
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
110
29
8
1
Host
Mouse
89
40
Clonality (Clone)
Monoclonal ()
Conjugate
This PML antibody is un-conjugated
5
5
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
107
47
41
39
26
24
14
6
6
4
3
3
2
1
1
1
1
Supplier
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Immunogen PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D12
Isotype IgG2a kappa
Specificity PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PML (PML Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 97517 DA
Gene ID 12406
Research Area Cancer, Transcription Factors, Signaling, Cell Structure
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (AA 411-510) (ABIN394032) Immunofluorescence of monoclonal antibody to PML on HeLa cell (antibody concentration...
Immunohistochemistry (IHC) image for anti-PML antibody (Promyelocytic Leukemia) (AA 411-510) (ABIN394032) anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody (Image 2)
Background publications Vincenzi, Perrone, Santini, Grosso, Silletta, Frezza, Rossi, Russo, Rabitti, Gebbia, Badalamenti, Casali, Muda, Dei Tos, Tonini: "PML down-regulation in soft tissue sarcomas." in: Journal of cellular physiology, Vol. 224, Issue 3, pp. 644-8, 2010 (PubMed).

Hasan, Ottone, Schlenk, Xiao, Wiemels, Mitra, Bernasconi, Di Raimondo, Stanghellini, Marco, Mays, Döhner, Sanz, Amadori, Grimwade, Lo-Coco: "Analysis of t(15;17) chromosomal breakpoint sequences in therapy-related versus de novo acute promyelocytic leukemia: association of DNA breaks with specific DNA motifs at PML and RARA loci." in: Genes, chromosomes & cancer, Vol. 49, Issue 8, pp. 726-32, 2010 (PubMed).

Mimura, Takahashi, Kawata, Akazawa, Inoue: "Two-step colocalization of MORC3 with PML nuclear bodies." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2014-24, 2010 (PubMed).

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Leavenworth, Groesch, Xin Hu, Malm, Torry, Abrams, Torry: "Dysregulation of promyelocytic leukemia (PML) protein expression in preeclamptic placentae." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 17, Issue 4, pp. 339-49, 2010 (PubMed).