Promyelocytic Leukemia (PML) (AA 411-510) antibody

Details for Product No. ABIN394032
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Antigen
Synonyms PML, MYL, PP8675, RNF71, TRIM19, 1200009E24Rik, AI661194, Trim19, RGD1562602
Epitope
AA 411-510
(12), (12), (9), (7), (7), (7), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(111), (43), (31), (24), (24), (24), (12), (1)
Host
Mouse
(99), (26)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(88), (42), (31), (30), (25), (21), (20), (10), (6), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394032
450.00 $
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Immunogen PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D12
Isotype IgG2a kappa
Specificity PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PML
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 97517 DA
Gene ID 12406
Research Area Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody Immunofluorescence of monoclonal antibody to PML on HeLa cell (antibody concentration 10 µg/mL). PML monoclonal antibody (M02), clone 1D12 Western Blot analysis of PML expression in Hela NE.
anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody (2) anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody (Image 2)
Background publications Leavenworth, Groesch, Xin Hu et al.: "Dysregulation of promyelocytic leukemia (PML) protein expression in preeclamptic placentae." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 17, Issue 4, pp. 339-49, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Mimura, Takahashi, Kawata et al.: "Two-step colocalization of MORC3 with PML nuclear bodies." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2014-24, 2010 (PubMed).

Hasan, Ottone, Schlenk et al.: "Analysis of t(15;17) chromosomal breakpoint sequences in therapy-related versus de novo acute promyelocytic leukemia: association of DNA breaks with specific DNA motifs at PML and RARA loci." in: Genes, chromosomes & cancer, Vol. 49, Issue 8, pp. 726-32, 2010 (PubMed).

Vincenzi, Perrone, Santini et al.: "PML down-regulation in soft tissue sarcomas." in: Journal of cellular physiology, Vol. 224, Issue 3, pp. 644-8, 2010 (PubMed).

Validation Images
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