Promyelocytic Leukemia (PML) antibody

Antigen: Promyelocytic Leukemia (PML)

Synonyms: MYL, RNF71, PP8675, TRIM19, Trim19, AI661194, 1200009E24Rik, RGD1562602, PML

Clonality: Monoclonal (1D12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394032

Additional Information

Alternative name: PML

Gene ID: 5371

Immunogen: PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1D12

Description: Other names: MYL, PP8675, RNF71, TRIM19 promyelocytic leukemia

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 97517 DA

Comments:

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to PML (AT3354a) on HeLa cell . [antibody concentration 10 ug/ml] PML monoclonal antibody (M02), clone 1D12 Western Blot analysis of PML expression in Hela NE ( Cat # L013V3 ).

anti-Promyelocytic Leukemia (PML) antibody (Image 2)

Publications

Product: Leavenworth, Groesch, Xin Hu et al.: "Dysregulation of promyelocytic leukemia (PML) protein expression in preeclamptic placentae." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 17, Issue 4, pp. 339-49, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Mimura, Takahashi, Kawata et al.: "Two-step colocalization of MORC3 with PML nuclear bodies." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2014-24, 2010 (PubMed).

Hasan, Ottone, Schlenk et al.: "Analysis of t(15;17) chromosomal breakpoint sequences in therapy-related versus de novo acute promyelocytic leukemia: association of DNA breaks with specific DNA motifs at PML and RARA loci." in: Genes, chromosomes & cancer, Vol. 49, Issue 8, pp. 726-32, 2010 (PubMed).

Vincenzi, Perrone, Santini et al.: "PML down-regulation in soft tissue sarcomas." in: Journal of cellular physiology, Vol. 224, Issue 3, pp. 644-8, 2010 (PubMed).