|+1 404 474 4654|
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Promyelocytic Leukemia (PML) antibody
|Synonyms||MYL, RNF71, PP8675, TRIM19, Trim19, AI661194, 1200009E24Rik, RGD1562602, PML|
Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: MYL, PP8675, RNF71, TRIM19 promyelocytic leukemia|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||97517 DA|
Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Leavenworth, Groesch, Xin Hu et al.: "Dysregulation of promyelocytic leukemia (PML) protein expression in preeclamptic placentae." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 17, Issue 4, pp. 339-49, 2010 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
Mimura, Takahashi, Kawata et al.: "Two-step colocalization of MORC3 with PML nuclear bodies." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2014-24, 2010 (PubMed).
Hasan, Ottone, Schlenk et al.: "Analysis of t(15;17) chromosomal breakpoint sequences in therapy-related versus de novo acute promyelocytic leukemia: association of DNA breaks with specific DNA motifs at PML and RARA loci." in: Genes, chromosomes & cancer, Vol. 49, Issue 8, pp. 726-32, 2010 (PubMed).
Vincenzi, Perrone, Santini et al.: "PML down-regulation in soft tissue sarcomas." in: Journal of cellular physiology, Vol. 224, Issue 3, pp. 644-8, 2010 (PubMed).