NFYC antibody

Antigen: NFYC

Clonality: Monoclonal (1D3)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394039

Additional Information

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Gene ID: 4802

Immunogen: NFYC (AAH05003, 14 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa  (Matching secondary antibodies)

Clone: 1D3

Description: Other names: CBFC, CBFC, DKFZp667G242, H1TF2A, HAP5, HSM, NFYC, hCBFC nuclear transcription factor Y, gamma

Molecular Weight: 50302 DA

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYC monoclonal antibody (M01), clone 1D3 (Cat # AT3043a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3. Lane 1: NFYC transfected lysate(37.2 KDa). Lane 2: Non-transfected lysate.

anti-NFYC antibody (Image 2)