Nuclear Transcription Factor Y, gamma (NFYC) antibody

Antigen: Nuclear Transcription Factor Y, gamma (NFYC)

Synonyms: HSM, CBFC, HAP5, CBF-C, NF-YC, H1TF2A, FLJ45775, DKFZp667G242, fb98f01, zgc:55514, wu:fb98f01, hsm, cbfc, hap5, cbf-c, nf-yc, h1tf2a, MGC75886, NFYC, MGC128196, Nfyc, DKFZp459H0131

Clonality: Monoclonal (1D3)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394039

Additional Information

Alternative name: NFYC

Gene ID: 4802

Immunogen: NFYC (AAH05003, 14 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 1D3

Description: Other names: CBFC, CBFC, DKFZp667G242, H1TF2A, HAP5, HSM, NFYC, hCBFC nuclear transcription factor Y, gamma

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: NFYC (AAH05003, 14 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 50302 DA

Comments:

Background: This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYC monoclonal antibody (M01), clone 1D3 (Cat # AT3043a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3. Lane 1: NFYC transfected lysate(37.2 KDa). Lane 2: Non-transfected lysate.

anti-Nuclear Transcription Factor Y, gamma (NFYC) antibody (Image 2)

Publications

Product: Horikawa, Wood, Yang et al.: "Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7956-62, 2008 (PubMed).

Yamanaka, Mizuarai, Eguchi et al.: "Expression levels of NF-Y target genes changed by CDKN1B correlate with clinical prognosis in multiple cancers." in: Genomics, Vol. 94, Issue 4, pp. 219-27, 2009 (PubMed).

Ceribelli, Benatti, Imbriano et al.: "NF-YC complexity is generated by dual promoters and alternative splicing." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34189-200, 2009 (PubMed).

Murai-Takeda, Shibata, Kurihara et al.: "NF-YC functions as a corepressor of agonist-bound mineralocorticoid receptor." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 8084-93, 2010 (PubMed).

Ichikawa, Koller, Padgett et al.: "Replication of previous genome-wide association studies of bone mineral density in premenopausal American women." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 25, Issue 8, pp. 1821-9, 2010 (PubMed).