Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) antibody

Antigen: Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3)

Synonyms: SIII, TCEB3A, FLJ38760, FLJ42849, 110kDa, Tceb3a, AA408125, dEloA, DmelCG6755, CG6755, tceb3a, zgc:56638, wu:fw66g10, TCEB3, MGC157191

Clonality: Monoclonal (1F3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394061

Additional Information

Alternative name: TCEB3

Gene ID: 6924

UniProt: NP_003189

Immunogen: TCEB3 (NP_003189, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F3

Description: Other names: SIII, TCEB3A transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TCEB3 (NP_003189, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 87230 DA

Comments:

Background: This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to TCEB3 (AT4181a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of TCEB3 expression in transfected 293T cell line by TCEB3 monoclonal antibody (M02), clone 1F3. Lane 1: TCEB3 transfected lysate(87.2 KDa). Lane 2: Non-transfected lysate.

anti-Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3) antibody (Image 2)

Publications

Product: Woo, Imm, Min et al.: "Structural and functional insights into the B30.2/SPRY domain." in: The EMBO journal, Vol. 25, Issue 6, pp. 1353-63, 2006 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Landa, Ruiz-Llorente, Montero-Conde et al.: "The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors." in: PLoS genetics, Vol. 5, Issue 9, pp. e1000637, 2009 (PubMed).