Carbonic Anhydrase VIII (CA8) (AA 40-140) antibody

Details for Product No. ABIN394065
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Antigen
Synonyms AW546993, Ca8, Cals, Cals1, Carp, wdl, zgc:110118, CA-VIII, CALS, CAMRQ3, CARP
Epitope
AA 40-140
(6), (5), (4), (2), (2), (1)
Reactivity
Human
(31), (25), (19), (14), (14), (12), (3), (2), (1), (1)
Host
Mouse
(33), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(17), (13), (10), (4), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394065
450.00 $
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Immunogen CA8 (NP_004047, 40 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F7
Isotype IgG3 kappa
Specificity CA8 (NP_004047, 40 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CA8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 32973 DA
Gene ID 12406
NCBI Accession NP_004047
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Carbonic Anhydrase VIII (CA8) (AA 40-140) antibody CA8 monoclonal antibody (M01), clone 1F7 Western Blot analysis of CA8 expression in A-549. Detection limit for recombinant GST tagged CA8 is approximately 3 ng/mL as a capture antibody.
anti-Carbonic Anhydrase VIII (CA8) (AA 40-140) antibody (2) anti-Carbonic Anhydrase VIII (CA8) (AA 40-140) antibody (Image 2)
Background publications Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Nishikata, Nishimori, Taniuchi et al.: "Carbonic anhydrase-related protein VIII promotes colon cancer cell growth." in: Molecular carcinogenesis, Vol. 46, Issue 3, pp. 208-14, 2007 (PubMed).

Mori, Kou, Sato et al.: "Nucleotide variations in genes encoding carbonic anhydrase 8 and 10 associated with femoral bone mineral density in Japanese female with osteoporosis." in: Journal of bone and mineral metabolism, Vol. 27, Issue 2, pp. 213-6, 2009 (PubMed).

Picaud, Muniz, Kramm et al.: "Crystal structure of human carbonic anhydrase-related protein VIII reveals the basis for catalytic silencing." in: Proteins, Vol. 76, Issue 2, pp. 507-11, 2009 (PubMed).

Türkmen, Guo, Garshasbi et al.: "CA8 mutations cause a novel syndrome characterized by ataxia and mild mental retardation with predisposition to quadrupedal gait." in: PLoS genetics, Vol. 5, Issue 5, pp. e1000487, 2009 (PubMed).

Validation Images
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