Carbonic Anhydrase VIII (CA8) antibody

Antigen: Carbonic Anhydrase VIII (CA8)

Synonyms: CALS, CARP, CA-VIII, MGC99509, MGC120502, wdl, Cals, Carp, Cals1, AW546993, ALRP, C-193, CVARP, MCARP, bA320F15.2, Alrp, Crap, Ca8, MGC105764, ANKRD1, MGC128138, ankrd1, zgc:171642, MGC108325, MGC116534

Clonality: Monoclonal (1F7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394065

Additional Information

Alternative name: CA8

Gene ID: 767

UniProt: NP_004047

Immunogen: CA8 (NP_004047, 40 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 1F7

Description: Other names: CAVIII, CALS, CARP, MGC120502, MGC99509 carbonic anhydrase VIII

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CA8 (NP_004047, 40 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 32973 DA

Comments:

Background: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form.

Synonyms: CALS, CARP, CA-VIII, MGC99509, MGC120502, wdl, Cals, Carp, Cals1, AW546993, ALRP, C-193, CVARP, MCARP, bA320F15.2, Alrp, Crap, Ca8, MGC105764, ANKRD1, MGC128138, ankrd1, zgc:171642, MGC108325, MGC116534

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

CA8 monoclonal antibody (M01), clone 1F7 Western Blot analysis of CA8 expression in A-549 ( Cat # L025V1 ). Detection limit for recombinant GST tagged CA8 is approximately 3ng/ml as a capture antibody.

anti-Carbonic Anhydrase VIII (CA8) antibody (Image 2)

Publications

Product: Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Nishikata, Nishimori, Taniuchi et al.: "Carbonic anhydrase-related protein VIII promotes colon cancer cell growth." in: Molecular carcinogenesis, Vol. 46, Issue 3, pp. 208-14, 2007 (PubMed).

Mori, Kou, Sato et al.: "Nucleotide variations in genes encoding carbonic anhydrase 8 and 10 associated with femoral bone mineral density in Japanese female with osteoporosis." in: Journal of bone and mineral metabolism, Vol. 27, Issue 2, pp. 213-6, 2009 (PubMed).

Picaud, Muniz, Kramm et al.: "Crystal structure of human carbonic anhydrase-related protein VIII reveals the basis for catalytic silencing." in: Proteins, Vol. 76, Issue 2, pp. 507-11, 2009 (PubMed).

Türkmen, Guo, Garshasbi et al.: "CA8 mutations cause a novel syndrome characterized by ataxia and mild mental retardation with predisposition to quadrupedal gait." in: PLoS genetics, Vol. 5, Issue 5, pp. e1000487, 2009 (PubMed).