TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A) (AA 1-101) antibody

Details for Product No. ABIN394070
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Antigen
Synonyms TAFI48, mTAFI48, MGC:17061, RAFI48, SL1
Epitope
AA 1-101
(10), (8), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(27), (10), (3), (2), (1)
Host
Mouse
(26), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(28), (16), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394070
450.00 $
Plus shipping costs $45.00

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Immunogen TAF1A (NP_005672, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F8
Isotype IgG2a kappa
Specificity TAF1A (NP_005672, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF1A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52676 DA
Gene ID 12406
NCBI Accession NP_005672
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A) (AA 1-101) antibody Detection limit for recombinant GST tagged TAF1A is approximately 0.1 ng/mL as a capture antibody.
Background publications Grandori, Gomez-Roman, Felton-Edkins et al.: "c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I." in: Nature cell biology, Vol. 7, Issue 3, pp. 311-8, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Young, Hassan, Pratap et al.: "Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2." in: Nature, Vol. 445, Issue 7126, pp. 442-6, 2007 (PubMed).

Greco-Stewart, Schissel, Pelchat: "The hepatitis delta virus RNA genome interacts with the human RNA polymerases I and III." in: Virology, Vol. 386, Issue 1, pp. 12-5, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

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