TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A) antibody

Details for Product No. ABIN394070
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Antigen
Synonyms TAFI48, mTAFI48, SL1, RAFI48, MGC:17061
Reactivity
Human
(21), (10), (3), (2), (1)
Host
Mouse
(20), (2)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(22), (10), (1), (1)
Pubmed 5 references available
Catalog no. ABIN394070
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen TAF1A (NP_005672, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F8
Isotype IgG2a kappa
Specificity TAF1A (NP_005672, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF1A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52676 DA
Gene ID 12406
UniProt NP_005672
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A) antibody Detection limit for recombinant GST tagged TAF1A is approximately 0.1 ng/mL as a capture antibody.
Background publications Grandori, Gomez-Roman, Felton-Edkins et al.: "c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I." in: Nature cell biology, Vol. 7, Issue 3, pp. 311-8, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1...." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Young, Hassan, Pratap et al.: "Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2." in: Nature, Vol. 445, Issue 7126, pp. 442-6, 2007 (PubMed).

Greco-Stewart, Schissel, Pelchat: "The hepatitis delta virus RNA genome interacts with the human RNA polymerases I and III." in: Virology, Vol. 386, Issue 1, pp. 12-5, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Hosts (20), (2)
Reactivities (21), (10), (3), (2), (1)
Applications (22), (10), (1), (1)
Epitopes (4), (2), (2), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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