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Four and A Half LIM Domains 5 (FHL5) (AA 1-285) antibody

Details for Product No. ABIN394080
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Antigen
Synonyms MGC82038, FHL5, act, ACT-1, ACT, RP3-393D12.2, dJ393D12.2, 1700027G07Rik, Act
Epitope
AA 1-285
(7), (5), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(25), (3), (3), (1), (1), (1), (1), (1)
Host
Mouse
(17), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(27), (13), (9), (4), (3), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394080
450.00 $
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Immunogen FHL5 (AAH21723, 1 a.a. ~ 285 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G12-D2
Isotype IgG2a kappa
Specificity FHL5 (AAH21723, 1 a.a. ~ 285 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name FHL5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 32720 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Alternative splicing results in multiple transcript variants encoding the same protein.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Four and A Half LIM Domains 5 (FHL5) (AA 1-285) antibody Immunofluorescence of monoclonal antibody to FHL5 on HepG2 cell (antibody concentration 10 µg/mL). Western Blot analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2. Lane 1: FHL5 transfected lysate(32.8 KDa). Lane 2: Non-transfected lysate.
anti-Four and A Half LIM Domains 5 (FHL5) (AA 1-285) antibody (2) anti-Four and A Half LIM Domains 5 (FHL5) (AA 1-285) antibody (Image 2)
Background publications Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Aston, Krausz, Laface et al.: "Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent." in: Human reproduction (Oxford, England), Vol. 25, Issue 6, pp. 1383-97, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Wu, Chen, Miao et al.: "Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis." in: FEBS letters, Vol. 584, Issue 13, pp. 2807-15, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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