Four and A Half LIM Domains 5 (FHL5) antibody

Antigen: Four and A Half LIM Domains 5 (FHL5)

Synonyms: ACT, FLJ33049, KIAA0776, dJ393D12.2, RP3-393D12.2, Act, 1700027G07Rik, MGC82038, FHL5, MGC134130, act

Clonality: Monoclonal (1G12-D2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394080

Additional Information

Alternative name: FHL5

Gene ID: 9457

Immunogen: FHL5 (AAH21723, 1 a.a. ~ 285 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1G12-D2

Description: Other names: ACT, FLJ33049, KIAA0776, dJ393D12.2 four and a half LIM domains 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FHL5 (AAH21723, 1 a.a. ~ 285 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 32720 DA

Comments:

Background: The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Alternative splicing results in multiple transcript variants encoding the same protein.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to FHL5 (AT2051a) on HepG2 cell . [antibody concentration 10 ug/ml] Western Blot analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2. Lane 1: FHL5 transfected lysate(32.8 KDa). Lane 2: Non-transfected lysate.

anti-Four and A Half LIM Domains 5 (FHL5) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Aston, Krausz, Laface et al.: "Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent." in: Human reproduction (Oxford, England), Vol. 25, Issue 6, pp. 1383-97, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Wu, Chen, Miao et al.: "Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis." in: FEBS letters, Vol. 584, Issue 13, pp. 2807-15, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).