Density-Regulated Protein (DENR) antibody

Antigen: Density-Regulated Protein (DENR)

Synonyms: DENR, DKFZp469K2322, denr, MGC84106, 1500003K04Rik, drp, drp1, smap-3, MGC79758, MGC107862, zgc:92629, zgc:113309, Denr, MGC138065, DRP, DRP1, SMAP-3

Clonality: Monoclonal (1H3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394092

Additional Information

Alternative name: DENR

Gene ID: 8562

UniProt: NP_003668

Immunogen: DENR (NP_003668, 1 a.a. ~ 82 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1H3

Description: Other names: DRP, DRP1, SMAP3 density-regulated protein

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DENR (NP_003668, 1 a.a. ~ 82 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 22092 DA

Comments:

Background: This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of DENR expression in transfected 293T cell line by DENR monoclonal antibody (M01), clone 1H3. Lane 1: DENR transfected lysate(22.092 KDa). Lane 2: Non-transfected lysate. Western blot analysis of DENR over-expressed 293 cell line, cotransfected with DENR Validated Chimera RNAi ( Cat # H00008562-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DENR monoclonal antibody (M01), clone 1H3 (Cat # AT1754a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Density-Regulated Protein (DENR) antibody (Image 2)

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Yonashiro, Ishido, Kyo et al.: "A novel mitochondrial ubiquitin ligase plays a critical role in mitochondrial dynamics." in: The EMBO journal, Vol. 25, Issue 15, pp. 3618-26, 2006 (PubMed).

Mazan-Mamczarz, Gartenhaus: "Post-transcriptional control of the MCT-1-associated protein DENR/DRP by RNA-binding protein AUF1." in: Cancer genomics & proteomics, Vol. 4, Issue 3, pp. 233-9, 2007 (PubMed).