T-Complex 1 (TCP1) antibody

Antigen: T-Complex 1 (TCP1)

Synonyms: CCT1, CCTa, D6S230E, CCT-alpha, TCP-1-alpha, Cct1-1P

Clonality: Monoclonal (2B2-D6)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394111

Additional Information

Alternative name: TCP1

Gene ID: 6950

Immunogen: TCP1 (AAH00665, 1 a.a. ~ 557 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2B2-D6

Description: Other names: CCTalpha, CCT1, CCTa, D6S230E, TCP1alpha t-complex 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TCP1 (AAH00665, 1 a.a. ~ 557 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 60344 DA

Comments:

Background: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to TCP1 (AT4198a) on HeLa cell . [antibody concentration 10 ug/ml] TCP1 monoclonal antibody (M01), clone 2B2-D6 Western Blot analysis of TCP1 expression in Hela ( Cat # L013V1 ).

anti-T-Complex 1 (TCP1) antibody (Image 2)

Publications

Product: Burgner, Davila, Breunis et al.: "A genome-wide association study identifies novel and functionally related susceptibility Loci for Kawasaki disease." in: PLoS genetics, Vol. 5, Issue 1, pp. e1000319, 2009 (PubMed).

Ridsdale, Tseu, Wang et al.: "Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells." in: American journal of respiratory cell and molecular biology, Vol. 43, Issue 1, pp. 74-87, 2010 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Mukherjee, Conway de Macario, Macario et al.: "Chaperonin genes on the rise: new divergent classes and intense duplication in human and other vertebrate genomes." in: BMC evolutionary biology, Vol. 10, pp. 64, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).