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ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 171-271)

Details for Product anti-ATF4 Antibody No. ABIN394112, Supplier: Log in to see
Antigen
  • atf-4
  • atf4
  • creb-2
  • creb2
  • taxreb67
  • txreb
  • wu:fj01c08
  • wu:fk30b04
  • zgc:85951
  • ATF4
  • ATF4C
  • sb:eu681
  • wu:fb08f07
  • zgc:171702
  • CREB-2
  • CREB2
  • TAXREB67
  • TXREB
  • Atf-4
  • C/ATF
Epitope
AA 171-271
51
36
28
28
16
13
13
9
8
7
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
250
74
72
5
3
2
2
1
1
1
Host
Mouse
209
45
15
Clonality (Clone)
Monoclonal ()
Conjugate
This ATF4 antibody is un-conjugated
11
11
10
9
9
9
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
186
106
92
40
24
23
22
14
6
4
4
2
1
Supplier
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Immunogen ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2B3
Isotype IgG1 kappa
Specificity ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ATF4 (ATF4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38590 DA
Gene ID 12406
NCBI Accession NP_001666
Research Area Transcription Factors, Signaling, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Restrictions For Research Use only
Concentration 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 171-271) (ABIN394112) Immunofluorescence of monoclonal antibody to ATF4 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 171-271) (ABIN394112) anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (AA 171-271) antibody (Image 2)
Background publications Liew, Bochenski, Kawamori et al.: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).

Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).

Rzymski, Milani, Pike et al.: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).

Ye, Kumanova, Hart et al.: "The GCN2-ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation." in: The EMBO journal, Vol. 29, Issue 12, pp. 2082-96, 2010 (PubMed).

Afonyushkin, Oskolkova, Philippova et al.: "Oxidized phospholipids regulate expression of ATF4 and VEGF in endothelial cells via NRF2-dependent mechanism: novel point of convergence between electrophilic and unfolded protein stress pathways." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 30, Issue 5, pp. 1007-13, 2010 (PubMed).