anti-ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 171-271)

Details for Product anti-ATF4 Antibody No. ABIN394112
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Antigen
Synonyms atf-4, atf4, creb-2, creb2, taxreb67, txreb, wu:fj01c08, wu:fk30b04, zgc:85951, ATF4, ATF4C, sb:eu681, wu:fb08f07, zgc:171702, CREB-2, CREB2, TAXREB67, TXREB, Atf-4, C/ATF
Epitope
AA 171-271
(47), (36), (34), (25), (15), (12), (9), (8), (7), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(232), (65), (58), (5), (3), (2), (1), (1), (1), (1)
Host
Mouse
(183), (46), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
This ATF4 antibody is un-conjugated
(11), (11), (10), (9), (9), (9), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(178), (103), (90), (43), (27), (24), (20), (13), (9), (7), (7), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Immunogen ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2B3
Isotype IgG1 kappa
Specificity ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ATF4 (ATF4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38590 DA
Gene ID 12406
NCBI Accession NP_001666
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Restrictions For Research Use only
Concentration 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (AA 171-271) antibody Immunofluorescence of monoclonal antibody to ATF4 on HeLa cell (antibody concentratio...
anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (AA 171-271) antibody (2) anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (AA 171-271) antibody (Image 2)
Background publications Liew, Bochenski, Kawamori et al.: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).

Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).

Rzymski, Milani, Pike et al.: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).

Ye, Kumanova, Hart et al.: "The GCN2-ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation." in: The EMBO journal, Vol. 29, Issue 12, pp. 2082-96, 2010 (PubMed).

Afonyushkin, Oskolkova, Philippova et al.: "Oxidized phospholipids regulate expression of ATF4 and VEGF in endothelial cells via NRF2-dependent mechanism: novel point of convergence between electrophilic and unfolded protein stress pathways." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 30, Issue 5, pp. 1007-13, 2010 (PubMed).

Validation Images
Catalog No. ABIN394112
450.00 $
Plus shipping costs $45.00
Quantity
Price
0.1 mg
450.00 $

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