Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) antibody

Antigen: Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4)

Synonyms: atf-4, MGC68539, zgc:85951, wu:fj01c08, wu:fk30b04, creb2, txreb, creb-2, MGC69565, taxreb67, ATF4, ATF4C, MGC126973, MGC179261, sb:eu681, wu:fb08f07, zgc:171702, CREB2

Clonality: Monoclonal (2B3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394112

Additional Information

Alternative name: ATF4

Gene ID: 468

UniProt: NP_001666

Immunogen: ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2B3

Description: Other names: CREB2, CREB2, TAXREB67, TXREBactivating transcription factor 4 (tax-responsive enhancer element B67)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ATF4 (NP_001666, 171 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 38590 DA

Comments:

Background: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ATF4 (AT1224a) on HeLa cell . [antibody concentration 10 ug/ml] ATF4 monoclonal antibody (M01), clone 2B3 Western Blot analysis of ATF4 expression in NIH/3T3 ( Cat # L018V1 ).

anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) antibody (Image 2)

Publications

Product: Afonyushkin, Oskolkova, Philippova et al.: "Oxidized phospholipids regulate expression of ATF4 and VEGF in endothelial cells via NRF2-dependent mechanism: novel point of convergence between electrophilic and unfolded protein stress pathways." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 30, Issue 5, pp. 1007-13, 2010 (PubMed).

Ye, Kumanova, Hart et al.: "The GCN2-ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation." in: The EMBO journal, Vol. 29, Issue 12, pp. 2082-96, 2010 (PubMed).

Rzymski, Milani, Pike et al.: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).

Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).

Liew, Bochenski, Kawamori et al.: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).