Aconitase 1, Soluble (ACO1) (AA 780-890) antibody

Details for Product No. ABIN394119
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Antigen
Synonyms ACO1, irp1, wu:fo75b03, EFE, ETH1, LEACO1, pTOM13, AI256519, Aco-1, Irebp, Irp1, ratireb, IREB1, IREBP, ACONS, IREBP1, IRP1, Acon1, Ratireb, Aco1
Epitope
AA 780-890
(8), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(49), (30), (27), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(45), (4), (2)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(33), (31), (27), (10), (7), (5), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394119
450.00 $
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Immunogen ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C1
Isotype IgG2a kappa
Specificity ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACO1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 98399 DA
Gene ID 12406
Research Area Translation Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Aconitase 1, Soluble (ACO1) (AA 780-890) antibody Detection limit for recombinant GST tagged ACO1 is approximately 0.3 ng/mL as a capture antibody.
Background publications Vashisht, Zumbrennen, Huang et al.: "Control of iron homeostasis by an iron-regulated ubiquitin ligase." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 718-21, 2009 (PubMed).

Salahudeen, Thompson, Ruiz et al.: "An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 722-6, 2009 (PubMed).

Zimmer, Lamb, Ebert et al.: "The connectivity map links iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2." in: Cancer research, Vol. 70, Issue 8, pp. 3071-9, 2010 (PubMed).

Popovic, Templeton: "Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding state." in: The Biochemical journal, Vol. 430, Issue 2, pp. 315-24, 2010 (PubMed).

Yang, Liang, Pfeiffer et al.: "Associations of 9p21 variants with cutaneous malignant melanoma, nevi, and pigmentation phenotypes in melanoma-prone families with and without CDKN2A mutations." in: Familial cancer, Vol. 9, Issue 4, pp. 625-33, 2010 (PubMed).

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