|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Aconitase 1, Soluble (ACO1) antibody
|Synonyms||IRP1, ACONS, IREB1, IREBP, IREBP1, Acon1, Ratireb, ACO1, MGC140553, wu:fo75b03, DKFZp469C1420, ratireb, MGC53330|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: IREB1, IREBP, IRP1 aconitase 1, soluble|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||98399 DA|
Background: Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged ACO1 is approximately 0.3ng/ml as a capture antibody.|
Vashisht, Zumbrennen, Huang et al.: "Control of iron homeostasis by an iron-regulated ubiquitin ligase." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 718-21, 2009 (PubMed).
Salahudeen, Thompson, Ruiz et al.: "An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 722-6, 2009 (PubMed).
Zimmer, Lamb, Ebert et al.: "The connectivity map links iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2." in: Cancer research, Vol. 70, Issue 8, pp. 3071-9, 2010 (PubMed).
Popovic, Templeton: "Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding state." in: The Biochemical journal, Vol. 430, Issue 2, pp. 315-24, 2010 (PubMed).
Yang, Liang, Pfeiffer et al.: "Associations of 9p21 variants with cutaneous malignant melanoma, nevi, and pigmentation phenotypes in melanoma-prone families with and without CDKN2A mutations." in: Familial cancer, Vol. 9, Issue 4, pp. 625-33, 2010 (PubMed).
|Hosts||Rabbit (28), Goat (3)|
|Reactivities||Human (23), Rat (Rattus) (14), Mouse (Murine) (12), Cow (Bovine) (3), Chicken (2), Dog (Canine) (2), Arabidopsis thaliana (1), Cat (Feline) (1), Rabbit (1), Tomato (Solanum lycopersicum) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (22), ELISA (20), Immunohistochemistry (IHC) (17), Immunofluorescence (IF) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1)|
|Epitopes||N-Term (4), AA 1-889 (1), C-Term (1), Internal Region (1), pSer138 (1), pSer711 (1)|