Aconitase 1, Soluble (ACO1) antibody

Antigen: Aconitase 1, Soluble (ACO1)

Synonyms: IRP1, ACONS, IREB1, IREBP, IREBP1, Acon1, Ratireb, ACO1, MGC140553, wu:fo75b03, DKFZp469C1420, ratireb, MGC53330

Clonality: Monoclonal (2C1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394119

Additional Information

Alternative name: ACO1

Gene ID: 48

Immunogen: ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2C1

Description: Other names: IREB1, IREBP, IRP1 aconitase 1, soluble

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ACO1 (AAH18103, 780 a.a. ~ 890 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 98399 DA

Comments:

Background: Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ACO1 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Vashisht, Zumbrennen, Huang et al.: "Control of iron homeostasis by an iron-regulated ubiquitin ligase." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 718-21, 2009 (PubMed).

Salahudeen, Thompson, Ruiz et al.: "An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 722-6, 2009 (PubMed).

Zimmer, Lamb, Ebert et al.: "The connectivity map links iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2." in: Cancer research, Vol. 70, Issue 8, pp. 3071-9, 2010 (PubMed).

Popovic, Templeton: "Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding state." in: The Biochemical journal, Vol. 430, Issue 2, pp. 315-24, 2010 (PubMed).

Yang, Liang, Pfeiffer et al.: "Associations of 9p21 variants with cutaneous malignant melanoma, nevi, and pigmentation phenotypes in melanoma-prone families with and without CDKN2A mutations." in: Familial cancer, Vol. 9, Issue 4, pp. 625-33, 2010 (PubMed).