AchE antibody

Details for Product No. ABIN394128
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Antigen
Reactivity
Human
(32), (20), (19), (2)
Host
Mouse
(31), (3), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(33), (15), (8), (5), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394128
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen ACHE (NP_000656, 515 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C3
Isotype IgG2a kappa
Specificity ACHE (NP_000656, 515 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 67796 DA
Gene ID 12406
UniProt NP_000656
Research Area Translation Factors, Cell Structure, Signaling
Application Notes Western blot = 1:500-1000
Comment

Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-AchE antibody ACHE monoclonal antibody (M02), clone 2C3 Western Blot analysis of ACHE expression in NIH/3T3.
Background publications Di Cristofaro, Silvy, Chiaroni et al.: "Single PCR multiplex SNaPshot reaction for detection of eleven blood group nucleotide polymorphisms: optimization, validation, and one year of routine clinical use." in: The Journal of molecular diagnostics : JMD, Vol. 12, Issue 4, pp. 453-60, 2010 (PubMed).

Cadieux, Broomfield, Kirkpatrick et al.: "Comparison of human and guinea pig acetylcholinesterase sequences and rates of oxime-assisted reactivation." in: Chemico-biological interactions, Vol. 187, Issue 1-3, pp. 229-33, 2010 (PubMed).

Howard, Hsu, Grzywacz et al.: "Evaluation of candidate genes for cholinesterase activity in farmworkers exposed to organophosphorus pesticides: association of single nucleotide polymorphisms in BCHE." in: Environmental health perspectives, Vol. 118, Issue 10, pp. 1395-9, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Hosts (31), (3), (2)
Reactivities (32), (20), (19), (2)
Applications (33), (15), (8), (5), (2), (1)
Epitopes (12), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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