EIF4ENIF1 antibody (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1) (AA 886-986)

Details for Product anti-EIF4ENIF1 Antibody No. ABIN394130, Supplier: Log in to see
Antigen
  • RGD1560908
  • eif4enif1
  • MGC80355
  • EIF4ENIF1
  • wu:fd15a05
  • zgc:101646
  • MGC147579
  • 4E-T
  • Clast4
  • 2610509L04Rik
  • A930019J01Rik
  • AA410001
  • AU021239
  • D11Ertd166e
  • eukaryotic translation initiation factor 4E nuclear import factor 1
  • Eif4enif1
  • EIF4ENIF1
  • eif4enif1
Epitope
AA 886-986
20
15
15
10
8
8
4
2
2
2
2
1
1
1
1
Reactivity
Human
91
27
25
8
8
6
4
4
4
3
3
2
2
1
Host
Mouse
57
20
14
Clonality (Clone)
Monoclonal ()
Conjugate
This EIF4ENIF1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
70
41
19
14
13
12
6
4
3
1
Options
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Immunogen EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C4
Isotype IgG2a kappa
Specificity EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EIF4ENIF1 (EIF4ENIF1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 108201 DA
Gene ID 12406
NCBI Accession NP_062817
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic, its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-EIF4ENIF1 antibody (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1) (AA 886-986) (ABIN394130) Western Blot analysis of EIF4ENIF1 expression in transfected 293T cell line by EIF4EN...
Background publications Suzuki, Minami, Suzuki, Abe, Zenno, Tsujimoto, Matsumoto, Minami: "The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and eIF4E-transporter into stress granules and association of eIF4E with eIF4G." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35597-604, 2009 (PubMed).

Venkatesan, Rual, Vazquez, Stelzl, Lemmens, Hirozane-Kishikawa, Hao, Zenkner, Xin, Goh, Yildirim, Simonis, Heinzmann, Gebreab, Sahalie, Cevik, Simon, de Smet, Dann, Smolyar, Vinayagam, Yu et al.: "An empirical framework for binary interactome mapping...." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Lee, Cho, Kim: "Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation." in: Biochemical and biophysical research communications, Vol. 369, Issue 4, pp. 1160-5, 2008 (PubMed).

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Denoeud, Kapranov, Ucla, Frankish, Castelo, Drenkow, Lagarde, Alioto, Manzano, Chrast, Dike, Wyss, Henrichsen, Holroyd, Dickson, Taylor, Hance, Foissac, Myers, Rogers, Hubbard, Harrow, Guigó et al.: "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions." in: Genome research, Vol. 17, Issue 6, pp. 746-59, 2007 (PubMed).