Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) (AA 886-986) antibody

Details for Product No. ABIN394130
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Antigen
Synonyms RGD1560908, eif4enif1, MGC80355, EIF4ENIF1, wu:fd15a05, zgc:101646, MGC147579, 4E-T, Clast4, 2610509L04Rik, A930019J01Rik, AA410001, AU021239, D11Ertd166e
Epitope
AA 886-986
(13), (8), (7), (4), (2), (1)
Reactivity
Human
(42), (3), (3), (2), (2), (2)
Host
Mouse
(26), (8), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(37), (20), (7), (6), (5), (4), (4), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394130
450.00 $
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Immunogen EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C4
Isotype IgG2a kappa
Specificity EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EIF4ENIF1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 108201 DA
Gene ID 12406
NCBI Accession NP_062817
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic, its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) (AA 886-986) antibody Western Blot analysis of EIF4ENIF1 expression in transfected 293T cell line by EIF4ENIF1 monoclonal antibody (M01), clone 2C4. Lane 1: EIF4ENIF1 transfected lysate(108.201 KDa). Lane 2: Non-transfected lysate.
Background publications Denoeud, Kapranov, Ucla et al.: "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions." in: Genome research, Vol. 17, Issue 6, pp. 746-59, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lee, Cho, Kim: "Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation." in: Biochemical and biophysical research communications, Vol. 369, Issue 4, pp. 1160-5, 2008 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping...." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Suzuki, Minami, Suzuki et al.: "The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and eIF4E-transporter into stress granules and association of eIF4E with eIF4G." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35597-604, 2009 (PubMed).

Validation Images
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