Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1)

Synonyms: 4E-T, Clast4, FLJ21601, FLJ26551, AA410001, AU021239, D11Ertd166e, 2610509L04Rik, A930019J01Rik, RGD1560908, eif4enif1, MGC80355, EIF4ENIF1, wu:fd15a05, zgc:101646, MGC147579

Clonality: Monoclonal (2C4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394130

Additional Information

Alternative name: EIF4ENIF1

Gene ID: 56478

UniProt: NP_062817

Immunogen: EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2C4

Description: Other names: 4E-T, Clast4, FLJ21601 eukaryotic translation initiation factor 4E nuclear import factor 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EIF4ENIF1 (NP_062817, 886 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 108201 DA

Comments:

Background: The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic, its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of EIF4ENIF1 expression in transfected 293T cell line by EIF4ENIF1 monoclonal antibody (M01), clone 2C4. Lane 1: EIF4ENIF1 transfected lysate(108.201 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Denoeud, Kapranov, Ucla et al.: "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions." in: Genome research, Vol. 17, Issue 6, pp. 746-59, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lee, Cho, Kim: "Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation." in: Biochemical and biophysical research communications, Vol. 369, Issue 4, pp. 1160-5, 2008 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Suzuki, Minami, Suzuki et al.: "The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and eIF4E-transporter into stress granules and association of eIF4E with eIF4G." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35597-604, 2009 (PubMed).